By Lawrence J. Shimkets (auth.), Dr. Frans J. de Bruijn, Dr. James R. Lupski, Dr. George M. Weinstock (eds.)
A wide variety of microbiologists, molecular biologists, and molecular evolutionary biologists will locate this new quantity of singular curiosity. It summarizes the current wisdom in regards to the constitution and balance of microbial genomes, and stories the concepts used to investigate and fingerprint them. Maps of roughly thirty very important microbes, in addition to articles at the building and appropriate positive factors of the maps are integrated. the quantity isn't really meant as a whole compendium of all info on microbial genomes, yet particularly makes a speciality of ways, equipment and sturdy examples of the research of small genomes.
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Additional resources for Bacterial Genomes: Physical Structure and Analysis
13:4751-4763. 22 / Karl Drlica and Conrad L. -C. and R. Beran. 1988. Multiple promoters for transcription of the E. coli DNA topoisomerase I gene and their regulation by DNA supercoiling. J. Mol. BioI. 202:735-742. , C. L. Woldringh, G. J. Brakenhoff, T. M. Van der Voort, and N. Nanninga. 1985. Confocal scanning light microscopy of the Escherichia coli nucleoid: comparison with phase-contrast and electron microscope images. J. Bacteriol. 161 :478483. M. and C. L. Woldringh. 1994. Nucleoid partitioning in Escherichia coli during steady-state growth and upon recovery from chloramphenicol treatment.
Until we determine which of these methods, if any, accurately reflects the intracellular condition, we will not know to what extent the nucleoid should be regarded as a distinct region separated from the cytoplasmic phase. Localization of Chromosomal Activities and Proteins Electron microscopic studies have revealed that transcription occurs at the periphery of the nucleoid, a point first made by autoradiographic detection of nascent transcripts in cells fixed with OS04 (Ryter and Chang, 1975).
Yet this perspective is not a static one. As we learn more about various bacterial genomes, the number of characterized bacterial insertion sequences (ISs) continues to increase. Examples are known from a wide range of Gram-negative and Gram-positive bacterial species as well as from the archaebacteria (reviewed by Galas and Chandler, 1989; Murphy, 1989; Charlebois and Doolittle, 1989). ISs are normal constituents not only of many bacterial chromosomes but also of some plasmids and bacteriophages.